Gel Electrophoresis Buffers and Diluents
Bioworld CHAPS Lysis Buffer (2X), 25 mL
This buffer is used for cell lysis and protein extraction.1x Concentration Contains:50mM Tris-HCl (pH 7.4)110mM NaCl5mM EDTA1% CHAPS.CHAPS is 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate Warning:For laboratory use only.Not fit for agricultural, household, clinical use or humanconsumption.
Hampton Research HR2-944-66/Index #66 (F06), 185 ml - 0.2 M Ammonium sulfate, 0.1 M BIS-TRIS pH 5.5, 25% w/v Polyethylene glycol 3,350
HR2-944-66/Index #66 (F06), 185 ml - 0.2 M Ammonium sulfate, 0.1 M BIS-TRIS pH 5.5, 25% w/v Polyethylene glycol 3,350. Please note items from this supplier are non-returnable.
Macherey-Nagel Buffer WASH-EF (1000 mL)
Buffer WASH-EF (1000 mL) Wash Buffer WASH-EF bottle of 1000 mL
Associates of Cape Cod Inc PYROSOL BUFFER W/PH 5.5ML 5PK
PYROSOL RECONSTITUTION BUFFER WITH pH INDICATOR, FOR USE WITH GEL CLOT ONLY, 5.5ML/VIAL, 5 VIALS/PACK, PYROCLEAR CERTIFIED
Sigma Aldrich Fine Chemicals Biosciences Sucrose | 57-50-1 | MFCD00006626 | 5 kg
Sucrose | Purity: ≥99.5% | Mol Wt: 342.30 | 57-50-1 | MFCD00006626 | 5 kg
Boston Bioproducts Inc Glycine Buffer (0.2 M, pH 3.0) - 500ml
The Glycine Buffer is widely used in various laboratory applications such as polyacrylamide gel electrophoresis as a running buffer, a transfer buffer for Western blotting, enzyme assays, and cell culture. This Buffer type serves as a tool for maintaining stable conditions during biochemical experiments in the pH range of 2.2 to 3.6. The Glycine buffer is an essential part of many types of protein purification procedures, including the purification and recovery of monoclonal antibodies.
Boston Bioproducts Inc Separating (Resolving) Buffer - 500ml
The Separating (Resolving) Buffer is a critical component in SDS-Polyacrylamide Gel Electrophoresis (PAGE), a laboratory technique that is used to separate protein molecules based on molecular weight. This buffer maintains the pH of the separating or resolving gel at 8.8, which is a higher pH level than that of the stacking gel.
LifeSensors UBITEST BUFFERS ONLY
The UbiTest kit identifies whether a target protein is polyubiquitinated and improves on traditional immunoprecipitation and Western blot methods, which often fail due to epitope masking, reduced antibody affinity, or changes in selectivity. By combining immunoprecipitation with broad‑spectrum DUB digestion, the assay reveals increased signal for the protein of interest after DUB treatment, confirming ubiquitination even when untreated samples show weak detection. To avoid variability caused by antibody–substrate interactions, UbiTest uses high‑affinity TUBEs to pull down polyubiquitinated proteins. These engineered ubiquitin‑binding domains have nanomolar affinity and TUBE1 binds all eight linkage types. The kit contains only the buffers needed for efficient elution of polyubiquitinated proteins.